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Glycose
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Wine RG-II (Sephadex G-75 fraction)a
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Celery RG-II (Q-Sepharose 1.5 M imidazole fraction)b
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Mol%
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MeFuc
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2.9 ± 0.1
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3.9 ± 0.4
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Rha
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16.3 ± 0.7
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14.9 ± 0.9
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Fuc
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1.8 ± 0.0
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2.9 ± 0.1
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MeXyl
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2.9 ± 0.2
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4.0 ± 0.4
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Ara
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17.6 ± 1.0
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14.1 ± 1.5
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Api
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3.6 ± 0.3
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3.9 ± 0.8
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AceA
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1.2 ± 0.1
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1.3 ± 0.3
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Gal
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17.9 ± 1.2
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10.7 ± 1.0
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Glc
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1.4 ± 0.1
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0.2 ± 0.0
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GalA
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29.2 ± 1.2
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39.9 ± 3.8
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GlcA
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5.2 ± 2.6
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4.1 ± 1.1
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- Quantification of neutral sugars and AceA was performed using alditol acetate derivatives. Quantification of uronic acids was performed using HPAEC-PAD. All values are expressed as a molar percentage and represent the average data from three replicates. See Additional file 1: Figure S2 for representative spectra
- aThe RG-II fraction isolated by SEC of the material solubilized by EPG treatment of celery petiole AIR (see Fig. 2 in the main text)
- bThe celery RG-II obtained by SEC was purified by anion-exchange chromatography. A solution of the celery RG-II (400 mg) in 10 mM imidazole–HCl pH 7 was applied to a column (15 cm × 2 cm; 47.1 mL column volume) of fast flow DEAE-Sepharose (Cytiva, USA). The column was eluted stepwise with 10 mM imidazole–HCl pH 7 (3 column volumes), 100 mM imidazole–HCl pH 7 (3 column volumes), and then with 1.5 M imidazole–HCl, pH 7 (4 column volumes). RG-II (~ 95% dimer, 295 mg) eluted with 1.5 M imidazole–HCl. A galactose-rich material (73 mg) eluted with 10 mM imidazole–HCl
