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Table 2 Summary of results obtained from parameter estimation analyses

From: Kinetic modeling of phosphorylase-catalyzed iterative β-1,4-glycosylation for degree of polymerization-controlled synthesis of soluble cello-oligosaccharides

Parameter PE18 PE19 PE20
Value Value Value
CdP reaction
 KiGlc1P, [mM] 0.32 0.32 0.32
 KGlc1P, [mM] 0.72 0.72 0.72
 KG2, [mM] 6.22 6.23 6.14
 KG3, [mM] 24.5 24.5 24.5
 KG4 (= KG5KG11), [mM] 98.6 99.1 58.5
 Keq 1.03 1.03 1.04
 k2, [−] 1.94 1.94 1.98
 k3, [−] 4.57 4.59 2.80
 k4, [−] 4.31 4.33 2.66
 k5 (= k6k10), [−] 1.56 1.56 1.00
Precipitation
 [G5]SL, [mM] 14.73 14.35 14.59
 [G6]SL, [mM] 2.68 2.68 2.68
 [G7]SL, [mM] 0.896 0.896 0.896
 [G8]SL, [mM] 0.1620 0.1674 0.1672
 [G9]SL, [mM] 0.0308 0.0313 0.0313
 [G10]SL, [mM] 0.00331 0.00333 0.00585
 [G11]SL, [mM] 0.001084 0.001087 0.000706
 [G12]SL, [mM] 0.000114 0.000196 0.000114