Modulating redox metabolism to improve isobutanol production in Shimwellia blattae

Table 2 Strains, genotype and plasmid used in the experimental work in this study

Strains	Genotype	References
Shimwellia blattae	Type strain	CIP 104942, DSMZ 4481
Escherichia coli DH5α	endA1, hsdR17, supE44, thi-1 recA1, gyrA (Nal^R), relA1 Δ(argF-lac), U169 depR, Φ80dlacd(lacZ) M15	[44]
Lactococcus lactis IL1403	Type strain	Provided by Dr. P. López (CIB-CSIC)

Plasmids	Genotype	References
pIZ1016	Gm^R, broad host range expression vector bearing lacI^q and Ptac. Rep (pBBR1MCS)	[46]
pIZ2	Extended pIZ1016 polylinker. EcoRI, ClaI, ScaI, SmaI (XmaI), SpeI, XvaI, SalI, PstI (SbfI), SphI, HindIII and SacI	Provided by Dr. G. Durante (CIB-CSIC)
pSEVA424	Sm^R/Sp^R, broad host range expression vector bearing lacI^q and Ptrc. Rep (RK2 replicative origin. oriV-trfA)	[47]
p424IbPSO	Sm^R/Sp^R. IbPSO operon (alsS-ilvC-ilvD-kdc) into pSEVA424	[22]
pIZadhA	Gm^R. adhA gene of L. lactis into pIZ2	This work
pIZpntAB	Gm^R. pntAB gene of E. coli into pIZ2	This work

ISSN: 1754-6834